Important! Why Is Oil Used With 100x Objective Lens

The most powerful lens of the light microscope is the 100x oil immersion objective. Oil immersion should be used between the slide and 100x objective lens this is a special oil that has the same refractive index as glass.

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Oil immersion is the technique of using a drop of oil to wet the top of the specimen or slide cover and the front of the objective lens.

Why is oil used with 100x objective lens. Because light is refracted every time it passes through a medium with a different refractive. When placed between the specimen and objective lens the oil forms a continuous lens. Furthermore why is immersion oil used with the 100x objective.

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